Evaluation of aberrant p16INK4α promoter CpG methylation and its application in Vietnamese breast cancers patients

DOAN THI, PHUONG THAO and LAO DUC, THUAN and LE HUYEN, AI THUY and TRUONG, KIM PHUONG (2015) Evaluation of aberrant p16INK4α promoter CpG methylation and its application in Vietnamese breast cancers patients. In: Fourth International Conference On Advances in Applied Science and Environmental Engineering - ASEE 2015, 26-27 September, 2015, Kuala Lumpur, Malaysia.

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The disruption of genetic materials as the aberrant methylation at CpG islands of tumor suppressor gene’s promoter, which is thought to be a driver epigenetic events of malignant cells. p16INK4α, its function is considered as an inhibitor of CDK4 and CDK6 that initiate the phosphorylation of RB (retinoblastoma tumor suppressor protein), has been identified as a tumor suppressor gene in many human cancers. In current study, methylation specific PCR (MSP) was carried out to evaluate the methylation status of p16INK4α from Vietnamese breast patients, in order to find out the methylation signature served as biomarker applied in prognosis, early detection and diagnosis of breast cancer in Vietnamese population. The results showed that the methylation frequency of p16INK4α promoter reached to 49.5% (47 of 95 breast cancer samples). In the case of non-cancer specimens, only 15% (3 of 20 non-cancer specimens) was methylation. According to clinical parameters, there were significant correlation between the p16INK4α aberrant methylation and the HER(-)p53(-) combination in breast cancer (p<0.05). Furthermore, the p16INK4α methylation increased the possibility to breast cancer with the high incidence via the odd ratio (OR = 5.55, p = 0.01). Simultaneously analyzed with hypermethylation of panel genes including BRCA1, Cyclin D2, GSTP1 and RASSF1A, it showed that the coverage of diagnosis, which based on evaluation of panel genes’ hypermethylation frequency, was 100% (with the methylation index (MI) ≥ 0.2). In conclusion, the hypermethylation at CpG islands of p16INK4α was the significant characteristic of breast cancer in Vietnamese population relied on the size of samples surveyed. Notably, co-analyzed with BRCA1, Cyclin D2, GSTP1 and RASSF1A, those hypermethylation characteristics could be served as significant biomarker for breast cancer.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: breast cancer, methylation, MSP, p16INK4α, Vietnamese breast cancer.
Depositing User: Mr. John Steve
Date Deposited: 26 Apr 2019 04:44
Last Modified: 26 Apr 2019 04:44
URI: http://publications.theired.org/id/eprint/1554

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