Electrochemical DNA Biosensor For Detection Of Porcine Oligonucleotides Using [Ru(bpy)2PIP]2+ Complex

HASLINA, AHMAD and LEE YOOK, HENG (2015) Electrochemical DNA Biosensor For Detection Of Porcine Oligonucleotides Using [Ru(bpy)2PIP]2+ Complex. In: Third International Conference on Advances in Bio-Informatics, Bio-Technology and Environmental Engineering- ABBE 2015, 26 - 27 May,2015, Birmingham B42 2SU, UNITED KINGDOM.

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Abstract

An electrochemical DNA biosensor for detection of porcine oligonucleotides based on ruthenium (II) complexruthenium(II) complex, [Ru(bpy)2(PIP)]2+, (bpy = 2,2’bipyridine, PIP = 2-phenylimidazo[4,5-f[[1,10-phenanthroline]) as label redox have been developed. The study was carried out by immobilization of porcine aminated DNA probes sequences on screen printed electrode (SPE) modified with succinimide-acrylic microspheres and [Ru(bpy)2(PIP)]2+ to detect DNA hybridization event. The electrochemical detection by redox active ruthenium (II) complex was measured by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The results indicate that the interaction of [Ru(bpy)2(PIP)]2+ with hybridization complementary DNA has higher response compared to single-stranded and mismatch complementary DNA. Under optimized condition, this porcine DNA biosensor shows linear response range towards target DNA within range of 1 x10 -5 uM to 1x10-13 uM

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: DNA biosensor, ruthenium, electrochemical
Depositing User: Mr. John Steve
Date Deposited: 30 Apr 2019 11:35
Last Modified: 30 Apr 2019 11:35
URI: http://publications.theired.org/id/eprint/1723

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