Cultivation of Medicinal Mushrooms Ganoderma lucidum and Grifola frondosa Mycelia and Polysaccharides in Bioreactors

ANDREJ, GREGORI and BOJANA, BOH and BRANKA, WRABER and JOZI, HABIJANIC and MARIN, BEROVIC and MIRJAN, SVAGELJ and MOJCA, PANKL (2015) Cultivation of Medicinal Mushrooms Ganoderma lucidum and Grifola frondosa Mycelia and Polysaccharides in Bioreactors. In: Third International Conference on Advances in Applied Science and Environmental Engineering - ASEE 2015, 11-12 APRIL 2015, Kuala Lumpur, Malaysia.

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Abstract

Original strains of Ganoderma lucidum ( MZKI G97) and Grifola frondosa (GF3) isolated from Slovenian forests were cultivated using submerged and solid state cultivation. In 14 days submerged Ganoderma lucidum fed batch cultivation extracellular (1,7 g/L) and intracellular (0.45 g/L) polysaccharide fractions were isolated, up to 17.0 g/L dry fungal biomass was produced, while in 28 days Grifola frondosa fed batch cultivation 3.65 g/L of extracellular and 1.30 g/L intracellular polysaccharide and 15.2 g/L dry biomass was produced. In Ganoderma lucidum solid state cultivation in 18 days 5.77 mg /g extracellular and 1.45 mg /g intracellular polysaccharide was produced while in Grifola frondosa in 38 days cultivation of 0.70 extracellular and 3.80 mg/g of intracellular polysaccharide were produced. The isolated polysaccharides were mainly -D-glucanes. Immunostimulatory effects of isolates were tested on induction of cytokine (TNF- , IFN- and IL12) synthesis in primary cultures of human mononuclear cells (PBMC) isolated from a buffy coat.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Ganoderma lucidum, Grifola frondosa, polysaccharides, induction of cytokines, bioreactors, submerged, solid state cultivation
Depositing User: Mr. John Steve
Date Deposited: 07 May 2019 10:14
Last Modified: 07 May 2019 10:14
URI: http://publications.theired.org/id/eprint/1998

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