Subcellular Level Resolution Fluorescence Measurements in Yeast Cells by Image Analysis

EVGENY, PUCHKOV (2018) Subcellular Level Resolution Fluorescence Measurements in Yeast Cells by Image Analysis. In: Eighth International Conference on Advances in Applied Science and Environmental Engineering - ASEE 2018, 03-04 February, 2018, Kuala Lumpur, Malaysia.

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Abstract

Computer image analysis algorithms of the ImageJ software (National Institutes of Health, USA; http://rsb.info.nih.gov/ij) have been tailored for subcellular microfluorimetry of the single Saccharomyces cerevisiae yeast cells. The algorithms were used for the quantitative analysis of digital images acquired by wide-field fluorescence microscopy combined with color digital photography aiming to investigate the intracellular distribution of anthracycline anticancer drug doxorubicin and to evaluate viscosity in the vacuoles of t he cells.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: computer image analysis, fluorimetry, fluorescence anisotropy, ImageJ, yeast cells, Saccharomyces cerevisiae, fluorescence microscopy, viscosity, vacuoles, anticancer drug, doxorubicin
Depositing User: Mr. John Steve
Date Deposited: 10 Mar 2019 09:19
Last Modified: 10 Mar 2019 09:19
URI: http://publications.theired.org/id/eprint/238

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