Antimicrobial and antimolluscal activities and assessment of the active constituents of plant latices and their sera

AHMED A. M., SHOREIT and MADY A. ISMAIL, ISMAIL and NADIA, H.MOHAMED (2014) Antimicrobial and antimolluscal activities and assessment of the active constituents of plant latices and their sera. In: International Conference on Advances In Applied Science and Environmental Engineering - ASEE 2014, 02 - 03 August, 2014, Kuala Lumpur, Malaysia.

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Abstract

The effect of three plant latices on some pathogenic bacteria, dermatophytes and phytopathogenic fungi, and two medically important species of molusca as well were studied. Trials for detection of the active compounds and/or enzymes in plant latex responsible for the antimicrobial activities were also carried out. The results revealed that the three plant latices have good antimicrobial activities on Pseudomonas aeruginosa, Escherichia coli, Trichophyton rubrum and Chrysosporium tropicum. Latex of Ficus nitida was the most effective against both bacterial and fungal species. Phytochemical analyses revealed the presence of several active compounds (glycosides, terpenoids and tannins) in the three plant latices, (saponins) in Euphorbia pulcherrima and Ficus elastica, (phenolic compounds and alkaloids) in E. pulcherrima and F. nitida, (flavonoids) in only F. nitida latex. The serum fraction of latex from both E. pulcherrima and F. nitida had protease, lipase, chitinase and cellulases (C1 and Cx) enzymes. Three different bands were obtained from the F. nitida serum when fractionated by preparative gel electrophoresis, and these bands were tested separately and showed antifungal activity. Casein incorporated into separation gel was degraded and appeared as a clear zone around one of the three bands (the protease band, the first one) and this indicates the presence of protease enzyme in the serum of F. nitida latex. The presence of such active compounds or enzymes in the latices may explain the antimicrobial activity of these latices. Latices of E. pulcherrima and F. nitida have potent molluscicidal activities on both medically important species of Biomphalaria alexandrina and Lymnaea truncatula.

Item Type: Conference or Workshop Item (Paper)
Depositing User: Mr. John Steve
Date Deposited: 27 May 2019 07:59
Last Modified: 27 May 2019 07:59
URI: http://publications.theired.org/id/eprint/2744

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