Production of Cellulolytic Enzyme from Paper Sludge by Geobacillus kaustophilus C1 and Its Application for Glucose Production from of Rice Straw

CHAKRIT, TACHAAPIKOON and KHANOK, RATANAKHANOKCHAI and PATTHRA, PASON and RATTIYA, WAEONUKUL and VANARAT, PHAKEENUYA (2017) Production of Cellulolytic Enzyme from Paper Sludge by Geobacillus kaustophilus C1 and Its Application for Glucose Production from of Rice Straw. In: Sixth International Conference on Advances in Applied Science and Environmental Technology - ASET 2017, 25-26 February 2017, Bangkok, Thailand.

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Abstract

The objective of this study was to produce cellulolytic enzyme from paper sludge (PS), a solid waste from pulp and paper manufacturer by Geobacillus kaustophilus strain C1. When PS was used as sole carbon source, cellulolytic enzyme, mainly β-glucosidase was produced by the strain C1 which exhibited good properties, high temperature and high glucose concentration tolerance. Cellulolytic enzyme from strain C1 was afterward applied to hydrolyze ammonia-treated rice straw (ARS) for glucose production. Glucose was produced as a major hydrolysis product. Degree of saccharification of cellulose in ARS to glucose was improved when crude enzyme of strain C1 was combined with commercial Trichoderma reesei cellulase (Celluclast 1.5 L) and revealed 78.1% glucan saccharification. Glucose from ARS can be subsequently used as raw material for production of value-added products in many industries.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Geobacillus kaustophilus, Glucose, βglucosidase, Paper sludge, Rice straw
Depositing User: Mr. John Steve
Date Deposited: 16 Mar 2019 11:57
Last Modified: 16 Mar 2019 11:57
URI: http://publications.theired.org/id/eprint/510

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