Non-invasive detection of LMP-1, LMP-2 (Epstein-Barr Latent membrane protein) load in the diagnosis of nasopharyngeal carcinoma in Vietnamese population based on nasopharyngeal brushing samples

BUI THI DUONG, ANH and HO TA, GIAP and LAO DUC, THUAN and LE HUYEN AI, THUY and LUONG BAO, DUY and NGO DONG, KHA and NGUYEN HAI, CHAU and NGUYEN HOANG, ANH TUAN and NGUYEN HUU, DUNG and TRAN NGOC, QUYNH (2016) Non-invasive detection of LMP-1, LMP-2 (Epstein-Barr Latent membrane protein) load in the diagnosis of nasopharyngeal carcinoma in Vietnamese population based on nasopharyngeal brushing samples. In: Fourth International Conference On Advances in Applied Science and Environmental Technology- ASET 2016, 07-08 May 2016, Bangkok, Thailand.

[img]
Preview
Text
20160516_110141.pdf - Published Version

Download (626kB) | Preview
Official URL: https://www.seekdl.org/conferences/paper/details/7...

Abstract

In Vietnam, there have been reported to be highly incident cancer of nasopharynx. Epstein-Barr virus (EBV) have been considered to be mainly associated with nasopharyngeal carcinoma (NPC), in which EBV-encoded latent protein 1, 2 (LMP-1, LMP2) were found to be important factor target to NPC development. Thus, it is important to explore whether a non-invasive method could be applicable to screen and early diagnosis of NPC. In current study, NP (nasopharyngeal) and non-NP brushing samples were collected consecutively from participants when they underwent NP biopsy at Cho Ray Hospital, HCMC, Vietnam. PCR assay were used to detection of LMP-1, LMP-2 and T-test statistical analysis were used to analyze its diagnosis value. NP-brushing samples from NPC patients showed frequency of 45.00% and 60.00% for LMP-1 and LMP-2, respectively. Conversely, none of any cases of non-NP brushing samples were found to be positive to target both/individual genes. Virtually, in combination of both two genes, the frequency was increased to 66.67% as well as the PI value (PI 0.5). The p value 0.05 (LMP-1: p = 0.037; LMP-2: p = 0.008; both: p = 0.004) was observed, that indicated the correlation of between the presence both LMP-1, LMP-2 and NPC. The specific value was 100.00% for each/both candidate genes. Moreover, a high odd ratio (OR) and relative risk (RR) were also calculated of each/both candidate genes. In the case of LMP-1, OR, RR were 25.6087 (95%CI = 01.3479 - 486.545, p = 0.0309), 14.4762 (95%CI = 0.9085 - 230.672, p = 0.00585), respectively; in the case of LMP-2, the OR, RR were 45.5882 (95%CI = 2.391 – 869.201, p = 0.0111), 19.0476.11 (95%CI = 1.2166 – 298.2256, p = 0.0358), respectively; both candidate genes, the OR and RR were 58.8000 (95%CI = 2.9081 – 1070.6992, p = 0.0076), 26.5714 (95%CI = 1.3193 – 320.7527, p = 0.0309), respectively. Due to those results, the detection of both LMP-1 and LMP-2 in NP brushing samples could be an effective supplement for NPC early diagnosis that being non-invasive and rapid, demonstrated great potential for screening the high-risk of NPC in Vietnamese population.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: non-invasive, LMP-1, LMP-2, nasopharyngeal carcinoma, NP brushing sample, Vietnamese population.
Depositing User: Mr. John Steve
Date Deposited: 23 Mar 2019 12:10
Last Modified: 23 Mar 2019 12:10
URI: http://publications.theired.org/id/eprint/830

Actions (login required)

View Item View Item