Immobilization Of Horseradish Peroxidase On Nanopolyaniline Surface Using Self-Assembly Techniques For Biosensor Applications

SITI AMIRA, OTHMAN and ZUR AIN AZREEN, ZAINUDDIN (2016) Immobilization Of Horseradish Peroxidase On Nanopolyaniline Surface Using Self-Assembly Techniques For Biosensor Applications. In: Fifth International Conference On Advances in Applied Science and Environmental Engineering - ASEE 2016, 12-13 March,2016, Kuala Lumpur, Malaysia.

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Abstract

This review paper describes the immobilization of horseradish peroxidase (HRP) on nanopolyaniline (PANI) by using self-assembly technique. Some chemically synthesized conductive polymers such as PANI were used as support for HRP immobilization. Enzymes immobilized were depend on their properties of immobilize of HRP such as optimum pH, thermal stability and storage stability and all these were investigated. The structure of PANI was characterized by FTIR, FESEM and UV-VIS. The structure of PANI depends on the pH of the phosphate buffer and the type of organic solvent. Thus, the formation of monolayer especially nano-particles of PANI was prepared by using Langmuir-Blodgett technique. The contribution in the development of the biosensor application for hydrogen peroxidase (H2O2) based on the HRP modified electrode was described.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Horseradish Peroxidase; Polyaniline;Immobilization; Enzymatic; Langmuir-Blodgett
Depositing User: Mr. John Steve
Date Deposited: 27 Mar 2019 12:07
Last Modified: 27 Mar 2019 12:07
URI: http://publications.theired.org/id/eprint/959

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