Transgenic Natural Killer Cells

ALIREZA, PIRAHMADIAN and SHAHRUDIN, ABDUL RAZAK (2016) Transgenic Natural Killer Cells. In: Fifth International Conference On Advances in Applied Science and Environmental Engineering - ASEE 2016, 12-13 March,2016, Kuala Lumpur, Malaysia.

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Abstract

Adaptive immunity, with its rearranging immunoglobulin and T-cell receptors, has caught most of the attention of current genetic and immunological research and overshadowed the importance of the receptors expressed by cells of the innate immune system. Natural Killer cells have evolved two main receptor systems to carry out their functions, both of them involving activating and inhibitory receptors include members from the Immunoglobulin-like superfamily as well as lectinlike receptors. Killer Immunoglobulin-like receptors (KIR) are polymorphic cell surface molecules present on Natural Killer (NK) cells which recognize classical HLA class I molecules and in doing so provide an alternative means of modulating the immune response to infected or tumoural cells. In this study we did the Separation of Natural killer cells (NK cells) from rat’s blood with the technique of LS column with the help of CD49 markers and Cultured of harvested NK cells in animal cells media (Dulbecco’s Modified Eagle’s Medium) with the help of animal cell culture technique. after pluralization of NK cells that was the Extraction of certain genes m RNA (Klra4,Klra8,KLRD1) of NK cells with the help of mRNA extraction technique .on the other hand we had to harvest hematopoietic stem cells from the thigh bone marrow of rats and culture them in to the special designed culture media for culturing stem cells (that would prevent the cell to form mature).at the end the main transfection would be done by transferring of protein synthesis system of Nk cells in to the harvested stem cells by the help of Lipofectamine

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Natural killer cells, Killer immunoglobulin-like receptors, human leukocyte antigen, major histocompatibility complex class I, Dulbecco’s Modified Eagle’s Medium.
Depositing User: Mr. John Steve
Date Deposited: 27 Mar 2019 12:07
Last Modified: 27 Mar 2019 12:07
URI: http://publications.theired.org/id/eprint/960

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